LOCUS       sequence-52339-e         610 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 610)
  TITLE     Chin Lab P85 plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 610)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLenti4/V5-DEST-P85(G376R)
FEATURES             Location/Qualifiers
     source          1..610
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    19..43
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             67..90
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ggaattctgc agatatcaac aagtttgtac aaaaaagcag gctccgcggc cgcccccttc
       61 accatggact ataaggacga tgatgacaaa tccatggcca tgagtgctga ggggtaccag
      121 tacagagcgc tgtatgatta taaaaaggaa agagaagaag atattgactt gcacttgggt
      181 gacatattga ctgtgaataa agggtcctta gtagctcttg gattcagtga tggacaggaa
      241 gccaggcctg aagaaattgg ctggttaaat ggctataatg aaaccacagg ggaaaggggg
      301 gactttccgg gaacttatgt agaatatatt ggaaggaaaa aaatctcgcc tcccacacca
      361 aagccccggc cacctcggcc tcttcctgtt gcaccaggtt cttcgaaaac tgaagcagat
      421 gttgaacaac aagctttgac tctcccggat cttgcagagc agtttgcccc tcctgacatt
      481 gccccgcctc ttcttatcaa gctcgtggaa gccattgaaa agaaaggtct ggaatgttca
      541 actctataca gaacacagag ctccagcaac ctggcagaat tacgacagct tcttgattgt
      601 gatacaccct
//
