LOCUS       sequence-52345-e         881 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 881)
  AUTHORS   Quayle SN, Chheda MG, Shukla SA, Wiedemeyer R, Tamayo P, Dewan RW, 
            Zhuang L, Huang-Hobbs E, Haidar S, Xiao Y, Ligon KL, Hahn WC, Chin L
  TITLE     Integrative functional genomics identifies RINT1 as a novel GBM 
            oncogene.
  JOURNAL   Neuro Oncol. 2012 Nov;14(11):1325-31. doi: 10.1093/neuonc/nos246. 
            Epub 2012 Oct 16.
  PUBMED    23074196
REFERENCE   2  (bases 1 to 881)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/neuonc/nos246"; journalName: "Neuro Oncol"; date: 
            "2012-11"; volume: "14"; issue: "11"; pages: "1325-31"
COMMENT     Sequence Label: pBabe-GW-GFP
FEATURES             Location/Qualifiers
     source          1..881
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    20..44
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(44..46,47..49,50..760)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(44..46,47..49,50..760)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(89..110)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(350..369)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     697..718
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    766..822
                     /label=MCS
                     /note="multiple cloning site"
     protein_bind    complement(840..864)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tgtggtggta cgtagatcaa caagtttgta caaaaaagtt ggcatggtga gcaagggcga
       61 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca
      121 caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa
      181 gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac
      241 ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa
      301 gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa
      361 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct
      421 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta
      481 caacagccac aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt
      541 caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa
      601 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc
      661 cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac
      721 cgccgccggg atcactctcg gcatggacga gctgtacaag tactcagatc tcgagctcaa
      781 gcttcgaatt ctgcagtcga cggtaccgcg ggcccgggat ccaccggatc tagataactg
      841 accaactttc ttgtacaaag tggttatgta tccatatgat g
//
