LOCUS       sequence-52358-e         861 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 861)
  AUTHORS   Rodrigo G, Landrain TE, Jaramillo A
  TITLE     De novo automated design of small RNA circuits for engineering 
            synthetic riboregulation in living cells.
  JOURNAL   Proc Natl Acad Sci U S A. 2012 Sep 18;109(38):15271-6. Epub 2012 Sep
            4.
  PUBMED    22949707
REFERENCE   2  (bases 1 to 861)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2012-09-18"; volume: "109(38)"; pages: 
            "15271-6. Epub 2012 Sep 4"
COMMENT     Sequence Label: pRAJ12m
FEATURES             Location/Qualifiers
     source          1..861
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             67..78
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     protein_bind    130..146
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             193..204
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 accttccata cgaactttga aacgcatgaa ctctttgata acgtcttcgg aggaagccat
       61 ctagtatttc tcctctttct ctagtggatc cgctaggatg catataaatg tgagcggata
      121 acattgacat tgtgagcgga taacaagata ctgagcacac ccagtatcat tctcttcttc
      181 ctgcccacgc ggaaagagga gaaaggtgta atgactagcc gtaaaggcga agagctgttc
      241 actggtgtcg tccctattct ggtggaactg gatggtgatg tcaacggtca taagttttcc
      301 gtgcgtggcg agggtgaagg tgacgcaact aatggtaaac tgacgctgaa gttcatctgt
      361 actactggta aactgccggt accttggccg actctggtaa cgacgctgac ttatggtgtt
      421 cagtgctttg ctcgttatcc ggaccatatg aagcagcatg acttcttcaa gtccgccatg
      481 ccggaaggct atgtgcagga acgcacgatt tcctttaagg atgacggcac gtacaaaacg
      541 cgtgcggaag tgaaatttga aggcgatacc ctggtaaacc gcattgagct gaaaggcatt
      601 gactttaaag aagacggcaa tatcctgggc cataagctgg aatacaattt taacagccac
      661 aatgtttaca tcaccgccga taaacaaaaa aatggcatta aagcgaattt taaaattcgc
      721 cacaacgtgg aggatggcag cgtgcagctg gctgatcact accagcaaaa cactccaatc
      781 ggtgatggtc ctgttctgct gccagacaat cactatctga gcacgcaaag cgttctgtct
      841 aaagatccga acgagaaacg c
//
