LOCUS       sequence-52369-e         910 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 910)
  AUTHORS   Filonov GS, Verkhusha VV
  TITLE     A Near-Infrared BiFC Reporter for In Vivo Imaging of Protein-Protein
            Interactions.
  JOURNAL   Chem Biol. 2013 Jul 23. pii: S1074-5521(13)00242-1. doi: 
            10.1016/j.chembiol.2013.06.009.
  PUBMED    23891149
REFERENCE   2  (bases 1 to 910)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Chem Biol. 
            2013 Jul 23. pii: S1074-5521(13)00242-1. doi: 
            10.1016/j.chembiol.2013.06.009."
COMMENT     Sequence Label: pC4-RHE-PAS
FEATURES             Location/Qualifiers
     source          1..910
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     5'UTR           87..126
                     /label=HSV TK 5'-UTR
                     /note="5' untranslated region from the herpes simplex virus
                     thymidine kinase gene"
     regulatory      141..150
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             564..842
                     /codon_start=1
                     /product="T2098L mutant of FKBP-rapamycin binding domain of
                     human FRAP"
                     /label=FRB*
                     /note="binds synthetic ligands such as AP21967"
                     /translation="ILWHEMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTL
                     KETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLLQAWDLYYHVFRRISK"
     CDS             849..875
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct ccatagaaga
       61 caccgggacc gatccagcct ccgggggatc ttggtggcgt gaaactcccg cacctcttcg
      121 gccagcgaat tccagaagcc gccaccatgg cttctagaat ggcggaagga tccgtcgcca
      181 ggcagcctga cctcttgacc tgcgacgatg agccgatcca tatccccggt gccatccaac
      241 cgcatggact gctgctcgcc ctcgccgccg acatgacgat cgttgccggc agcgacaacc
      301 ttcccgaact caccggactg gcgatcggcg ccctgatcgg ccgctctgcg gccgatgtct
      361 tcgactcgga gacgcacaac cgtctgacga tcgccttggc cgagcccggg gcggccgtcg
      421 gagcaccgat cactgtcggc ttcacgatgc gaaaggacgc aggcttcatc ggctcctggc
      481 atcgccatga tcagctcatc ttcctcgagc tcgagcctcc ccagcgggga ggcgggggaa
      541 gcggcggagg cgggtcctct agaatcctct ggcatgagat gtggcatgaa ggcctggaag
      601 aggcatctcg tttgtacttt ggggaaagga acgtgaaagg catgtttgag gtgctggagc
      661 ccttgcatgc tatgatggaa cggggccccc agactctgaa ggaaacatcc tttaatcagg
      721 cctatggtcg agatttaatg gaggcccaag agtggtgcag gaagtacatg aaatcaggga
      781 atgtcaagga cctcctccaa gcctgggacc tctattatca tgtgttccga cgaatctcaa
      841 agactagtta tccgtacgac gtaccagact acgcataaga aaagtgagga tcctgagaac
      901 ttcagggtga
//
