LOCUS       sequence-52370-e         775 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 775)
  AUTHORS   Filonov GS, Verkhusha VV
  TITLE     A Near-Infrared BiFC Reporter for In Vivo Imaging of Protein-Protein
            Interactions.
  JOURNAL   Chem Biol. 2013 Jul 23. pii: S1074-5521(13)00242-1. doi: 
            10.1016/j.chembiol.2013.06.009.
  PUBMED    23891149
REFERENCE   2  (bases 1 to 775)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Chem Biol. 
            2013 Jul 23. pii: S1074-5521(13)00242-1. doi: 
            10.1016/j.chembiol.2013.06.009."
COMMENT     Sequence Label: pC4EN-F1-GAFm
FEATURES             Location/Qualifiers
     source          1..775
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     5'UTR           85..124
                     /label=HSV TK 5'-UTR
                     /note="5' untranslated region from the herpes simplex virus
                     thymidine kinase gene"
     CDS             154..180
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             160..186
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             202..222
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             229..549
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
ORIGIN
        1 agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc atagaagaca
       61 ccgggaccga tccagcctcc gggggatctt ggtggcgtga aactcccgca cctcttcggc
      121 cagcgaattc cagaagcgcg tatggcttct agctatcctt atgacgtgcc tgactatgcc
      181 agcctgggag gaccttctag tcctaagaag aagagaaagg tgtctagagg agtgcaggtg
      241 gaaaccatct ccccaggaga cgggcgcacc ttccccaagc gcggccagac ctgcgtggtg
      301 cactacaccg ggatgcttga agatggaaag aaatttgatt cctcccggga cagaaacaag
      361 ccctttaagt ttatgctagg caagcaggag gtgatccgag gctgggaaga aggggttgcc
      421 cagatgagtg tgggtcagag agccaaactg actatatctc cagattatgc ctatggtgcc
      481 actgggcacc caggcatcat cccaccacat gccactctcg tcttcgatgt ggagcttcta
      541 aaactggaaa ctagtggagg cgggggaagc ggcggaggcg ggtcccctcc ccagcgggac
      601 gtcgccgagc cgcaggcgtt cttccgccgc accaacagcg ccatccgccg cctgcaggcc
      661 gccgaaacct tggaaagcgc ctgcgccgcc gcggcgcaag aggtgcggaa gattaccggc
      721 tacgatcggg tgatgatcta tcgcttcgcc tccgacttca gcggcgaagt gatcg
//
