LOCUS       sequence-52374-e         837 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 837)
  AUTHORS   Rodrigo G, Landrain TE, Jaramillo A
  TITLE     De novo automated design of small RNA circuits for engineering 
            synthetic riboregulation in living cells.
  JOURNAL   Proc Natl Acad Sci U S A. 2012 Sep 18;109(38):15271-6. Epub 2012 Sep
            4.
  PUBMED    22949707
REFERENCE   2  (bases 1 to 837)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2012-09-18"; volume: "109(38)"; pages: 
            "15271-6. Epub 2012 Sep 4"
COMMENT     Sequence Label: pRAJ31
FEATURES             Location/Qualifiers
     source          1..837
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             65..76
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     protein_bind    308..326
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    333..351
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    390..406
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             453..464
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 cttccatacg aactttgaaa cgcatgaact ctttgataac gtcttcggag gaagccatct
       61 agtatttctc ctctttctct agtggatccg ctagcatcga tgcgaaaaaa ccccgccgaa
      121 gcggggtttt ttgcggctag caaaaaaaga tcgtagccat gtagcaaata gctactaaga
      181 ccaatacagt gacagcggat agggacttaa ttgcggatga gacgacggta aagcttaata
      241 ttgatcctag caaccattta ccacataact acgacccagg cgctggacgt ttgatgtgtg
      301 ctcagtatct ctatcactga tagggatgtc aatctctatc actgataggg acctaggatg
      361 catataaatg tgagcggata acattgacat tgtgagcgga taacaagata ctgagcacag
      421 tggatatgct ttacctcttg gtgagacgac ataaagagga gaaacataat atgactagcc
      481 gtaaaggcga agagctgttc actggtgtcg tccctattct ggtggaactg gatggtgatg
      541 tcaacggtca taagttttcc gtgcgtggcg agggtgaagg tgacgcaact aatggtaaac
      601 tgacgctgaa gttcatctgt actactggta aactgccggt accttggccg actctggtaa
      661 cgacgctgac ttatggtgtt cagtgctttg ctcgttatcc ggaccatatg aagcagcatg
      721 acttcttcaa gtccgccatg ccggaaggct atgtgcagga acgcacgatt tcctttaagg
      781 atgacggcac gtacaaaacg cgtgcggaag tgaaatttga aggcgatacc ctggtaa
//
