LOCUS       sequence-52378-e         839 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 839)
  AUTHORS   Rodrigo G, Landrain TE, Jaramillo A
  TITLE     De novo automated design of small RNA circuits for engineering 
            synthetic riboregulation in living cells.
  JOURNAL   Proc Natl Acad Sci U S A. 2012 Sep 18;109(38):15271-6. Epub 2012 Sep
            4.
  PUBMED    22949707
REFERENCE   2  (bases 1 to 839)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2012-09-18"; volume: "109(38)"; pages: 
            "15271-6. Epub 2012 Sep 4"
COMMENT     Sequence Label: pRAJ31m
FEATURES             Location/Qualifiers
     source          1..839
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             64..75
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     protein_bind    127..143
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             190..201
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 ttccatacga actttgaaac gcatgaactc tttgataacg tcttcggagg aagccatcta
       61 gtatttctcc tctttctcta gtggatccgc taggatgcat ataaatgtga gcggataaca
      121 ttgacattgt gagcggataa caagatactg agcacagtgg atatgcttta cctcttggtg
      181 agacgacata aagaggagaa acataatatg actagccgta aaggcgaaga gctgttcact
      241 ggtgtcgtcc ctattctggt ggaactggat ggtgatgtca acggtcataa gttttccgtg
      301 cgtggcgagg gtgaaggtga cgcaactaat ggtaaactga cgctgaagtt catctgtact
      361 actggtaaac tgccggtacc ttggccgact ctggtaacga cgctgactta tggtgttcag
      421 tgctttgctc gttatccgga ccatatgaag cagcatgact tcttcaagtc cgccatgccg
      481 gaaggctatg tgcaggaacg cacgatttcc tttaaggatg acggcacgta caaaacgcgt
      541 gcggaagtga aatttgaagg cgataccctg gtaaaccgca ttgagctgaa aggcattgac
      601 tttaaagaag acggcaatat cctgggccat aagctggaat acaattttaa cagccacaat
      661 gtttacatca ccgccgataa acaaaaaaat ggcattaaag cgaattttaa aattcgccac
      721 aacgtggagg atggcagcgt gcagctggct gatcactacc agcaaaacac tccaatcggt
      781 gatggtcctg ttctgctgcc agacaatcac tatctgagca cgcaaagcgt tctgtctaa
//
