LOCUS       sequence-52381-e         637 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 637)
  AUTHORS   Rodrigo G, Landrain TE, Jaramillo A
  TITLE     De novo automated design of small RNA circuits for engineering 
            synthetic riboregulation in living cells.
  JOURNAL   Proc Natl Acad Sci U S A. 2012 Sep 18;109(38):15271-6. Epub 2012 Sep
            4.
  PUBMED    22949707
REFERENCE   2  (bases 1 to 637)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2012-09-18"; volume: "109(38)"; pages: 
            "15271-6. Epub 2012 Sep 4"
COMMENT     Sequence Label: pRAJ11-12
FEATURES             Location/Qualifiers
     source          1..637
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             4..15
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      complement(41..68)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(84..155)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    251..269
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    276..294
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     RBS             402..413
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 gtatttctcc tctttctcta gtggatccgc tagctataaa cgcagaaagg cccacccgaa
       61 ggtgagccag tgtgactcta gtagagagcg ttcaccgaca aacaacagat aaaacgaaag
      121 gcccagtctt tcgactgagc ctttcgtttt atttgatgcc tggcctaggg cctcgcataa
      181 atctgtcaca gcatcaacgt ttccgctgaa ctgtgacaga ctcacacaat caaccctccc
      241 gtgctcagta tctctatcac tgatagggat gtcaatctct atcactgata gggaatgcat
      301 gctaggatgc atataaatgt gagcggataa cattgacatt gtgagcggat aacaggatac
      361 tgagcacacc cagtatcatt ctcttcttcc tgcccacgcg gaaagaggag aaaggtgtaa
      421 tgactagccg taaaggcgaa gagctgttca ctggtgtcgt ccctattctg gtggaactgg
      481 atggtgatgt caacggtcat aagttttccg tgcgtggcga gggtgaaggt gacgcaacta
      541 atggtaaact gacgctgaag ttcatctgta ctactggtaa actgccggta ccttggccga
      601 ctctggtaac gacgctgact tatggtgttc agtgctt
//
