LOCUS       sequence-52390-e         899 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 899)
  AUTHORS   Rodrigo G, Landrain TE, Jaramillo A
  TITLE     De novo automated design of small RNA circuits for engineering 
            synthetic riboregulation in living cells.
  JOURNAL   Proc Natl Acad Sci U S A. 2012 Sep 18;109(38):15271-6. Epub 2012 Sep
            4.
  PUBMED    22949707
REFERENCE   2  (bases 1 to 899)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2012-09-18"; volume: "109(38)"; pages: 
            "15271-6. Epub 2012 Sep 4"
COMMENT     Sequence Label: pSTC0
FEATURES             Location/Qualifiers
     source          1..899
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      64..107
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(111..129)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        197..301
                     /gene="bla"
                     /label=AmpR promoter
     CDS             486..864
                     /label=ISS
                     /note="immunostimulatory sequence from the AmpR gene; 
                     contains unmethylated CpG dinucleotides in the context of 
                     5'-AACGTT-3' (Sato et al., 1996)"
     primer_bind     complement(520..539)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 gaattgggac aactccagtg aaaagttctt ctcctttacg gctagcagcg gccgcgaatt
       61 ccagaaatca tccttagcga aagctaagga ttttttttat ctgaaattct gcctcgtgat
      121 acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac
      181 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
      241 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
      301 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
      361 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
      421 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
      481 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
      541 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
      601 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
      661 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
      721 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
      781 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
      841 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctag
//
