LOCUS       sequence-52391-e         961 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 961)
  AUTHORS   Shaw RJ, Kosmatka M, Bardeesy N, Hurley RL, Witters LA, DePinho RA, 
            Cantley LC
  TITLE     The tumor suppressor LKB1 kinase directly activates AMP-activated 
            kinase and regulates apoptosis in response to energy stress.
  JOURNAL   Proc Natl Acad Sci U S A 2004 Mar 9;101(10):3329-35. Epub 2004 Feb 
            25.
  PUBMED    14985505
REFERENCE   2  (bases 1 to 961)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A"; date: "2004-03-9"; volume: "101(10)"; pages: 
            "3329-35. Epub 2004 Feb 25"
COMMENT     Sequence Label: pBABE-FLAG-LKB1
FEATURES             Location/Qualifiers
     source          1..961
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      12..21
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             21..44
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 atctaagctt agccaccatg gactacaaag acgatgacga taaagcaagg gatccactag
       61 taacggccgc cagtgtgctg gaattctcca gcatggaggt ggtggacccg cagcagctgg
      121 gcatgttcac ggagggcgag ctgatgtcgg tgggtatgga cacgttcatc caccgcatcg
      181 actccaccga ggtcatctac cagccgcgcc gcaagcgggc caagctcatc ggcaagtacc
      241 tgatggggga cctgctgggg gaaggctctt acggcaaggt gaaggaggtg ctggactcgg
      301 agacgctgtg caggagggcc gtcaagatcc tcaagaagaa gaagttgcga aggatcccca
      361 acggggaggc caacgtgaag aaggaaattc aactactgag gaggttacgg cacaaaaatg
      421 tcatccagct ggtggatgtg ttatacaacg aagagaagca gaaaatgtat atggtgatgg
      481 agtactgcgt gtgtggcatg caggaaatgc tggacagcgt gccggagaag cgtttcccag
      541 tgtgccaggc ccacgggtac ttctgtcagc tgattgacgg cctggagtac ctgcatagcc
      601 agggcattgt gcacaaggac atcaagccgg ggaacctgct gctcaccacc ggtggcaccc
      661 tcaaaatctc cgacctgggc gtggccgagg cactgcaccc gttcgcggcg gacgacacct
      721 gccggaccag ccagggctcc ccggctttcc agccgcccga gattgccaac ggcctggaca
      781 ccttctccgg cttcaaggtg gacatctggt cggctggggt caccctctac aacatcacca
      841 cgggtctgta ccccttcgaa ggggacaaca tctacaagtt gtttgagaac atcgggaagg
      901 ggagctacgc catcccgggc gactgtggcc ccccgctctc tgacctgctg aaagggatgc
      961 t
//
