LOCUS       sequence-52393-e         867 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 867)
  AUTHORS   Rodrigo G, Landrain TE, Jaramillo A
  TITLE     De novo automated design of small RNA circuits for engineering 
            synthetic riboregulation in living cells.
  JOURNAL   Proc Natl Acad Sci U S A. 2012 Sep 18;109(38):15271-6. Epub 2012 Sep
            4.
  PUBMED    22949707
REFERENCE   2  (bases 1 to 867)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2012-09-18"; volume: "109(38)"; pages: 
            "15271-6. Epub 2012 Sep 4"
COMMENT     Sequence Label: pSTC0r1
FEATURES             Location/Qualifiers
     source          1..867
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    89..105
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     misc_feature    144..165
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      168..211
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(215..233)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        301..405
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(624..643)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 caagaattgg gacaactcca gtgaaaagtt cttctccttt acggctagtc atctagtttt
       61 tcctctttct ctagttgtgc tcagtatctt gttatccgct cacaatgtca atgttatccg
      121 ctcacattta tgctagcatg catctctaga agcggccgcg aattccagaa atcatcctta
      181 gcgaaagcta aggatttttt ttatctgaaa ttctgcctcg tgatacgcct atttttatag
      241 gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg
      301 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
      361 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
      421 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
      481 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
      541 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
      601 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
      661 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
      721 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
      781 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
      841 ctaaccgctt ttttgcacaa catgggg
//
