LOCUS       sequence-52396-e         844 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 844)
  AUTHORS   Bahler J, Wu JQ, Longtine MS, Shah NG, McKenzie A 3rd, Steever AB, 
            Wach A, Philippsen P, Pringle JR
  TITLE     Heterologous modules for efficient and versatile PCR-based gene 
            targeting in Schizosaccharomyces pombe.
  JOURNAL   Yeast. 1998 Jul;14(10):943-51.
  PUBMED    9717240
REFERENCE   2  (bases 1 to 844)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast."; 
            date: "1998-07"; volume: "14(10)"; pages: "943-51"
COMMENT     Sequence Label: pFA6a-kanMX6-P41nmt-3HA
FEATURES             Location/Qualifiers
     source          1..844
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(52..239)
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     160..184
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     CDS             complement(272..361)
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
ORIGIN
        1 tacgctgcag gtcgacggat ctatattacc ctgttatccc tagcggatct gccggtagag
       61 gtgtggtcaa taagagcgac ctcatactat acctgagaaa gcaacctgac ctacaggaaa
      121 gagttactca agaataagaa ttttcgtttt aaaacctaag agtcacttta aaatttgtat
      181 acacttattt tttttataac ttatttaata ataaaaatca taaatcataa gaaattcgct
      241 tatttagaag tggcgcgcct cagcactgag cagcgtaatc tggaacgtca tatggatagg
      301 atcctgcata gtccgggacg tcatagggat agcccgcata gtcaggaaca tcgtatgggt
      361 aaaagatgtt aattaaagac atgatttaac aaagcgacta taagtcagaa agtgagaatg
      421 agattgaaat aattaattca acttattcaa ttgatgatat gccaggattc ctcttccttt
      481 atcttcaatg tttcgattaa caggtccatt atccggtttt tagtttcgct gcacattgcc
      541 gaatgacaat cggattcctt taagctttgc ttttgccctg ttttctctcc ccagtaatcg
      601 ttgggtgact gaaccatcca gtagccgaga gttacgtttt tcctgattta ataaatatcc
      661 atatctattt gtctcgtggt gttttacagc aactaaccga aattactatt ttgcatcatc
      721 caaccataaa gagacaacaa taattctaga aaacatataa caagtgtttt tttctattta
      781 cgtctcatac gcaaagtcca ctttaccatg ttttggaaac ttgtacccaa ccattcattt
      841 tcga
//
