LOCUS       sequence-52397-e         884 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 884)
  AUTHORS   Bahler J, Wu JQ, Longtine MS, Shah NG, McKenzie A 3rd, Steever AB, 
            Wach A, Philippsen P, Pringle JR
  TITLE     Heterologous modules for efficient and versatile PCR-based gene 
            targeting in Schizosaccharomyces pombe.
  JOURNAL   Yeast. 1998 Jul;14(10):943-51.
  PUBMED    9717240
REFERENCE   2  (bases 1 to 884)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast."; 
            date: "1998-07"; volume: "14(10)"; pages: "943-51"
COMMENT     Sequence Label: pFA6a-kanMX6-P3nmt1-3HA
FEATURES             Location/Qualifiers
     source          1..884
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             127..216
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      249..436
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(304..328)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     promoter        complement(512..530)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(513..530)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(611..630)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     730..752
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(790..808)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gatatataaa ggaagaggaa tcctggcata tcatcaattg aataagttga attaattatt
       61 tcaatctcat tctcactttc tgacttatag tcgctttgtt aaatcatgtc tttaattaac
      121 atcttttacc catacgatgt tcctgactat gcgggctatc cctatgacgt cccggactat
      181 gcaggatcct atccatatga cgttccagat tacgctgctc agtgctgagg cgcgccactt
      241 ctaaataagc gaatttctta tgatttatga tttttattat taaataagtt ataaaaaaaa
      301 taagtgtata caaattttaa agtgactctt aggttttaaa acgaaaattc ttattcttga
      361 gtaactcttt cctgtaggtc aggttgcttt ctcaggtata gtatgaggtc gctcttattg
      421 accacacctc taccggcaga tccgctaggg ataacagggt aatatagatc cgtcgacctg
      481 cagcgtacga agcttcagct ggcggccgcg ttctatagtg tcacctaaat cgtatgtgta
      541 tgatacataa ggttatgtat taattgtagc cgcgttctaa cgacaatatg tccatatggt
      601 gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa
      661 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg
      721 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga
      781 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
      841 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacc
//
