LOCUS       sequence-52400-e         787 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 787)
  AUTHORS   Rodrigo G, Landrain TE, Jaramillo A
  TITLE     De novo automated design of small RNA circuits for engineering 
            synthetic riboregulation in living cells.
  JOURNAL   Proc Natl Acad Sci U S A. 2012 Sep 18;109(38):15271-6. Epub 2012 Sep
            4.
  PUBMED    22949707
REFERENCE   2  (bases 1 to 787)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2012-09-18"; volume: "109(38)"; pages: 
            "15271-6. Epub 2012 Sep 4"
COMMENT     Sequence Label: pRAJ11
FEATURES             Location/Qualifiers
     source          1..787
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             54..65
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     protein_bind    112..128
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    167..185
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    192..210
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     terminator      306..377
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      393..420
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     misc_feature    432..453
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      456..499
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(503..521)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        589..693
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 attgggacaa ctccagtgaa aagttcttct cctttacggc tagtcattca taatttctcc
       61 tctttaacgg gaggattgaa gaagtgaaat tatgcgaggg tgctcagtat cttgttatcc
      121 gctcacaatg tcaatgttat ccgctcacat ttatgctagc atgcattccc tatcagtgat
      181 agagattgac atccctatca gtgatagaga tactgagcac gggagggttg attgtgtgag
      241 tctgtcacag ttcagcggaa acgttgatgc tgtgacagat ttatgcgagg ccctaggcca
      301 ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt
      361 tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg
      421 tttatagcta gctctagaag cggccgcgaa ttccagaaat catccttagc gaaagctaag
      481 gatttttttt atctgaaatt ctgcctcgtg atacgcctat ttttataggt taatgtcatg
      541 ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
      601 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
      661 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
      721 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
      781 aaagtaa
//
