LOCUS       sequence-52402-e         851 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 851)
  AUTHORS   Rodrigo G, Landrain TE, Jaramillo A
  TITLE     De novo automated design of small RNA circuits for engineering 
            synthetic riboregulation in living cells.
  JOURNAL   Proc Natl Acad Sci U S A. 2012 Sep 18;109(38):15271-6. Epub 2012 Sep
            4.
  PUBMED    22949707
REFERENCE   2  (bases 1 to 851)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2012-09-18"; volume: "109(38)"; pages: 
            "15271-6. Epub 2012 Sep 4"
COMMENT     Sequence Label: pRAJ21
FEATURES             Location/Qualifiers
     source          1..851
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    114..130
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    169..187
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    194..212
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     terminator      292..363
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      379..406
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     misc_feature    418..439
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      442..485
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(489..507)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        575..679
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gaattgggac aactccagtg aaaagttctt ctcctttacg gctagtcata accaatatct
       61 cctctttact ggaggaagca aagaagagat actaggcagg ggtgctcagt atcttgttat
      121 ccgctcacaa tgtcaatgtt atccgctcac atttatgcta gcatgcattc cctatcagtg
      181 atagagattg acatccctat cagtgataga gatactgagc actggaggag agagcgatcc
      241 tagttctcac tcaaagaagg gtaggactag gcagggccct aggccaggca tcaaataaaa
      301 cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct
      361 ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta tagctagctc
      421 tagaagcggc cgcgaattcc agaaatcatc cttagcgaaa gctaaggatt ttttttatct
      481 gaaattctgc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc
      541 ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
      601 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
      661 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
      721 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
      781 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
      841 ccttgagagt t
//
