LOCUS       sequence-52405-e         843 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 843)
  AUTHORS   Rodrigo G, Landrain TE, Jaramillo A
  TITLE     De novo automated design of small RNA circuits for engineering 
            synthetic riboregulation in living cells.
  JOURNAL   Proc Natl Acad Sci U S A. 2012 Sep 18;109(38):15271-6. Epub 2012 Sep
            4.
  PUBMED    22949707
REFERENCE   2  (bases 1 to 843)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2012-09-18"; volume: "109(38)"; pages: 
            "15271-6. Epub 2012 Sep 4"
COMMENT     Sequence Label: pRAJ21m
FEATURES             Location/Qualifiers
     source          1..843
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    113..129
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     misc_feature    161..182
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      185..228
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(232..250)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        318..422
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(641..660)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 aattgggaca actccagtga aaagttcttc tcctttacgg ctagtcataa ccaatatctc
       61 ctctttactg gaggaagcaa agaagagata ctaggcaggg gtgctcagta tcttgttatc
      121 cgctcacaat gtcaatgtta tccgctcaca tttatgctag ctctagaagc ggccgcgaat
      181 tccagaaatc atccttagcg aaagctaagg atttttttta tctgaaattc tgcctcgtga
      241 tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca
      301 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
      361 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
      421 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
      481 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
      541 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
      601 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
      661 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
      721 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
      781 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
      841 tcg
//
