LOCUS       sequence-52407-e         874 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 874)
  AUTHORS   Rodrigo G, Landrain TE, Jaramillo A
  TITLE     De novo automated design of small RNA circuits for engineering 
            synthetic riboregulation in living cells.
  JOURNAL   Proc Natl Acad Sci U S A. 2012 Sep 18;109(38):15271-6. Epub 2012 Sep
            4.
  PUBMED    22949707
REFERENCE   2  (bases 1 to 874)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2012-09-18"; volume: "109(38)"; pages: 
            "15271-6. Epub 2012 Sep 4"
COMMENT     Sequence Label: pRAJ22
FEATURES             Location/Qualifiers
     source          1..874
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    113..129
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    168..186
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    193..211
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     terminator      315..386
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      402..429
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     misc_feature    441..462
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      465..508
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(512..530)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        598..702
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aattgggaca actccagtga aaagttcttc tcctttacgg ctagtcatta aacttatctc
       61 ctctttaacg tgtgggccaa agaagaaaca agttgacggc gtgctcagta tcttgttatc
      121 cgctcacaat gtcaatgtta tccgctcaca tttatgctag catgcattcc ctatcagtga
      181 tagagattga catccctatc agtgatagag atactgagca cgacgtgtta gcgggccaaa
      241 gattgctagt tgacggctga tgcttgagta tgatagtagt ttatactcag gtgtactaag
      301 cctaggccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta
      361 tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc
      421 ctttctgcgt ttatagctag ctctagaagc ggccgcgaat tccagaaatc atccttagcg
      481 aaagctaagg atttttttta tctgaaattc tgcctcgtga tacgcctatt tttataggtt
      541 aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc
      601 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
      661 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
      721 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
      781 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
      841 ctggatctca acagcggtaa gatccttgag agtt
//
