LOCUS       sequence-52412-e         882 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 882)
  AUTHORS   Rodrigo G, Landrain TE, Jaramillo A
  TITLE     De novo automated design of small RNA circuits for engineering 
            synthetic riboregulation in living cells.
  JOURNAL   Proc Natl Acad Sci U S A. 2012 Sep 18;109(38):15271-6. Epub 2012 Sep
            4.
  PUBMED    22949707
REFERENCE   2  (bases 1 to 882)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2012-09-18"; volume: "109(38)"; pages: 
            "15271-6. Epub 2012 Sep 4"
COMMENT     Sequence Label: pRAJ23
FEATURES             Location/Qualifiers
     source          1..882
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    113..129
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    168..186
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    193..211
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     terminator      323..394
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      410..437
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     misc_feature    449..470
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      473..516
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(520..538)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        606..710
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aattgggaca actccagtga aaagttcttc tcctttacgg ctagtcatac attacatctc
       61 ctctttaaga gggtgcctaa agaagaaata caaccgacac gtgctcagta tcttgttatc
      121 cgctcacaat gtcaatgtta tccgctcaca tttatgctag catgcattcc ctatcagtga
      181 tagagattga catccctatc agtgatagag atactgagca cggagggtcg cctaaagcga
      241 tttgtttgct cccgcgttca tacctaggat gtgtggagca gatcggtcat aagggatatg
      301 atcgatatcc taggccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt
      361 tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc
      421 gggtgggcct ttctgcgttt atagctagct ctagaagcgg ccgcgaattc cagaaatcat
      481 ccttagcgaa agctaaggat tttttttatc tgaaattctg cctcgtgata cgcctatttt
      541 tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa
      601 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
      661 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
      721 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
      781 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
      841 acatcgaact ggatctcaac agcggtaaga tccttgagag tt
//
