LOCUS       sequence-52413-e         867 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 867)
  AUTHORS   Rodrigo G, Landrain TE, Jaramillo A
  TITLE     De novo automated design of small RNA circuits for engineering 
            synthetic riboregulation in living cells.
  JOURNAL   Proc Natl Acad Sci U S A. 2012 Sep 18;109(38):15271-6. Epub 2012 Sep
            4.
  PUBMED    22949707
REFERENCE   2  (bases 1 to 867)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2012-09-18"; volume: "109(38)"; pages: 
            "15271-6. Epub 2012 Sep 4"
COMMENT     Sequence Label: pRAJ23m
FEATURES             Location/Qualifiers
     source          1..867
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    112..128
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     misc_feature    160..181
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      184..227
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(231..249)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        317..421
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(640..659)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 attgggacaa ctccagtgaa aagttcttct cctttacggc tagtcataca ttacatctcc
       61 tctttaagag ggtgcctaaa gaagaaatac aaccgacacg tgctcagtat cttgttatcc
      121 gctcacaatg tcaatgttat ccgctcacat ttatgctagc tctagaagcg gccgcgaatt
      181 ccagaaatca tccttagcga aagctaagga ttttttttat ctgaaattct gcctcgtgat
      241 acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac
      301 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
      361 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
      421 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
      481 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
      541 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
      601 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
      661 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
      721 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
      781 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
      841 cggaggaccg aaggagctaa ccgcttt
//
