LOCUS       sequence-52416-e         731 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Korneel Hens, Jean-Daniel Feuz, Alina Isakova, Antonina Lagovitina, 
            Andreas Massouras, Julien Bryois, Patrick Callaerts, Susan E. 
            Celniker, Bart Deplancke
  TITLE     Automated protein-DNA interaction screening of Drosophila regulatory
            elements
  JOURNAL   Nat Methods. 2011 Oct 30;8(12):1065-70.
  PUBMED    22037703
REFERENCE   2  (bases 1 to 731)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods."; date: "2011-10-30"; volume: "8(12)"; pages: "1065-70"
COMMENT     Sequence Label: KHBD00744
FEATURES             Location/Qualifiers
     source          1..731
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    74..98
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(585..684)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(700..717)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(700..716)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(709..731)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 aataatgatt ttattttgac tgatagtgac ctgttcgttg caacaaattg ataagcaatg
       61 ctttcttata atgccaactt tgtacaagaa agttggcaat gcgtggaagg tcttcatctt
      121 tttattgcgt ctactgaagt cctggtcgag gtcttcgtcg tcggcctcct cgctgcccac
      181 ttcctcgtcg ctgagaaatt cgctgaactg atccccattt gaactctcca gtgcttgtat
      241 ctgcttagct attatgttgt tttgcctctt gatctcttcg atgtctttct ggttttcact
      301 gatcttccgc ctcatcgtct gtatgcattc ggtggtcttt ttcaggattt gagctcgact
      361 cgccttttcg cccttgagtg tgggcaccgc ctcacgcagg ttggtgaagc tctccttgat
      421 gtgatcgcgc cgccttcgct ccaaagcgtt gtggtgcgcc cgcttttcgg cctgggtgaa
      481 attggcggtg tttgtgtgtc gcgaggatcc caagccagtg tccgagtccc cgtcctcgtc
      541 actttcgatg tctatgtcgc ggtcgtcgtc gctcatactc atggtgccaa cttttttgta
      601 caaagttggc attataaaaa agcattgctc atcaatttgt tgcaacgaac aggtcactat
      661 cagtcaaaat aaaatcatta tttggggccc gagcttaaga ctggccgtcg ttttacaacg
      721 tcgtgactgg g
//
