LOCUS       sequence-52425-e         889 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 889)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 889)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: YWHAB
FEATURES             Location/Qualifiers
     source          1..889
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             759..779
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             786..803
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(856..874)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
ORIGIN
        1 cctctgaaat attttgttta ctttaagaag gagatataca tatgacaatg gataaaagtg
       61 agctggtaca gaaagccaaa ctcgctgagc aggctgagcg atatgatgat atggctgcag
      121 ccatgaaggc agtcacagaa caggggcatg aactctccaa cgaagagaga aatctgctct
      181 ctgttgccta caagaatgtg gtaggcgccc gccgctcttc ctggcgtgtc atctccagca
      241 ttgagcagaa aacagagagg aatgagaaga agcagcagat gggcaaagag taccgtgaga
      301 agatagaggc agaactgcag gacatctgca atgatgttct ggagctgttg gacaaatatc
      361 ttattcccaa tgctacacaa ccagaaagta aggtgttcta cttgaaaatg aaaggagatt
      421 attttaggta tctttctgaa gtggcatctg gagacaacaa acaaaccact gtgtcgaact
      481 cccagcaggc ttaccaggaa gcatttgaaa ttagtaagaa agaaatgcag cctacacacc
      541 caattcgtct tggtctggca ctaaatttct cagtctttta ctatgagatt ctaaactctc
      601 ctgaaaaggc ctgtagcctg gcaaaaacgg catttgatga agcaattgct gaattggata
      661 cgctgaatga agagtcttat aaagacagca ctctgatcat gcagttactt agggacaatc
      721 tcactctgtg gacatcggaa aaccagggag acgaaggaga gaacctgtac ttccaatccc
      781 tcgagcacca ccaccaccac cactgagatc cggctgctaa caaagcccga aaggaagctg
      841 agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggc
//
