LOCUS       sequence-52426-e         876 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 876)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 876)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: YWHAB
FEATURES             Location/Qualifiers
     source          1..876
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(27..44)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(51..71)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     RBS             796..818
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    837..853
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 ttcgggcttg tagcagccgg atctcagtgg tggtggtggt ggtgctcgag ggattggaag
       61 tacaggttct ctccttcgtc tccctggttt tccgatgtcc acagagtgag attgtcccta
      121 agtaactgca tgatcagagt gctgtcttta taagactctt cattcagcgt atccaattca
      181 gcaattgctt catcaaatgc cgtttttgcc aggctacagg ccttttcagg agagtttaga
      241 atctcatagt aaaagactga gaaatttagt gccagaccaa gacgaattgg gtgtgtaggc
      301 tgcatttctt tcttactaat ttcaaatgct tcctggtaag cctgctggga gttcgacaca
      361 gtggtttgtt tgttgtctcc agatgccact tcagaaagat acctaaaata atctcctttc
      421 attttcaagt agaacacctt actttctggt tgtgtagcat tgggaataag atatttgtcc
      481 aacagctcca gaacatcatt gcagatgtcc tgcagttctg cctctatctt ctcacggtac
      541 tctttgccca tctgctgctt cttctcattc ctctctgttt tctgctcaat gctggagatg
      601 acacgccagg aagagcggcg ggcgcctacc acattcttgt aggcaacaga gagcagattt
      661 ctctcttcgt tggagagttc atgcccctgt tctgtgactg ccttcatggc tgcagccata
      721 tcatcatatc gctcagcctg ctcagcgagt ttggctttct gtaccagctc acttttatcc
      781 attgtcatat gtatatctcc ttcttaaagt taaacaaaat tatttctaga ggggaattgt
      841 tatccgctca caaattcccc tatagtgagt cgtatt
//
