LOCUS       sequence-52428-e         854 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 854)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 854)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: YWHAG
FEATURES             Location/Qualifiers
     source          1..854
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(28..45)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(52..72)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     RBS             821..843
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 ttcgggcttg ttagcagccg gatctcagtg gtggtggtgg tggtgctcga gggattggaa
       61 gtacaggttc tcattgttgc cttcgccgcc atcgtcgtcc tgctggtcgc tcgtccagag
      121 cgtgaggttg tcgcggagga gctgcatgat gagcgtggag tccttgtagg agtcctcgtt
      181 gagggtgtca agctcggcga tggcgtcgtc gaacgcggtc ttggccaagt ggcacgcttg
      241 ctctggggcg ttctggatct catagtagaa gacggagtag ttaagagcca ggcctaatcg
      301 gatggggtgg gtgggctgca tgtgctcttt gctgatctcg tgggcttcgc tgtaggcctt
      361 ctcagaggac tccaccaccg tcgccctttt ctctccggtg gccacttcag ccaggtagcg
      421 gtagtagtcc cctttcatct tcaggtagaa cactttgctc tcgtactggg tctcgctgca
      481 attcttgatc aggtagttat ccagcaggct cagcacatcc tggcacacag cctccaactc
      541 cttctctatc ttctcccggt acgcacggac catctcaatc ttcttctcat tgccgtctgc
      601 agatgtcttc tgctcaatgc tactgatgac cctccaggaa gagcggcgtg cccccacaac
      661 gttcttgtag gccacagaca gaaggtttcg ttcctcattc gacagtggct cattcagctc
      721 tgtcacgttc ttcatggccg cggccatgtc gtcgtagcgc tccgcctgct cggccagccg
      781 ggctttctgc accagttgct cgcggtccac catatgtata tctccttctt aaagttaaac
      841 aaaattattt ctag
//
