LOCUS       sequence-52430-e         861 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 861)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 861)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: AKR7A2
FEATURES             Location/Qualifiers
     source          1..861
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             43..60
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             85..105
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 ctagaataat tttgtttact ttaagaagga gatatacata tgcaccatca tcatcatcat
       61 tcttctggtg tagatctggg taccgagaac ctgtacttcc aatccatgtc ccggccaccg
      121 ccaccgcggg tcgcctcggt gctgggcacc atggagatgg ggcgccgcat ggacgcgccc
      181 gccagcgccg cggccgtgcg cgcctttctg gagcgcggcc acaccgaact ggacacggcc
      241 ttcatgtaca gcgacggcca gtccgagacc atcctgggcg gcctggggct cgggctgggc
      301 ggtggcgact gcagagtgaa aattgccacc aaggccaacc cttgggatgg aaaatcacta
      361 aagcctgaca gtgtccggtc ccagctggag acgtcattga agaggctgca gtgtccccaa
      421 gtggacctct tctacctaca cgcacctgac cacggcaccc cggtggaaga gacgctgcat
      481 gcctgccagc ggctgcacca ggagggcaag ttcgtggagc ttggcctctc caactatgct
      541 agctgggaag tggccgagat ctgtaccctc tgcaagagca atggctggat cctgcccact
      601 gtgtaccagg gcatgtacaa cgccaccacc cggcaggtgg aaacggagct cttcccctgc
      661 ctcaggcact ttggactgag gttctatgcc tacaaccctc tggctggggg cctgctgact
      721 ggcaagtaca agtatgagga caaggacggg aaacagcctg tgggccgctt ctttgggaat
      781 agctgggctg agacctacag gaatcgcttc tggaaggagc accacttcga ggccattgcg
      841 ttggtggaga aggccctgca g
//
