LOCUS       sequence-52433-e         880 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 880)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: FDPS
FEATURES             Location/Qualifiers
     source          1..880
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             13..35
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             54..71
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             84..104
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 cctctagaat attttgttta actttaagaa ggagatatac catgggcagc agccatcatc
       61 atcatcatca cagcagcggc agagaaaact tgtatttcca gggccatatg aacggagacc
      121 agaattcaga tgtttatgcc caagaaaagc aggatttcgt tcagcacttc tcccagatcg
      181 ttagggtgct gactgaggat gagatggggc acccagagat aggagatgct attgcccggc
      241 tcaaggaggt cctggagtac aatgccattg gaggcaagta taaccggggt ttgacggtgg
      301 tagtagcatt ccgggagctg gtggagccaa ggaaacagga tgctgatagt ctccagcggg
      361 cctggactgt gggctggtgt gtggaactgc tgcaagcttt cttcctggtg gcagatgaca
      421 tcatggattc atcccttacc cgccggggac agatctgctg gtatcagaag ccgggcgtgg
      481 gtttggatgc catcaatgat gctaacctcc tggaagcatg tatctaccgc ctgctgaagc
      541 tctattgccg ggagcagccc tattacctga acctgatcga gctcttcctg cagagttcct
      601 atcagactga gattgggcag accctggacc tcctcacagc cccccagggc aatgtggatc
      661 ttgtcagatt cactgaaaag aggtacaaat ctattgtcaa gtacaagaca gctttctact
      721 ccttctacct tcctatagct gcagccatgt acatggcagg aattgatggc gagaaggagc
      781 acgccaatgc caagaagatc ctgctggaga tgggggagtt ctttcagatt caggatgatt
      841 accttgacct ctttggggac cccagtgtga ccggcaaaat
//
