LOCUS       sequence-52437-e         845 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 845)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 845)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: PIM1
FEATURES             Location/Qualifiers
     source          1..845
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             6..28
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             39..56
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             81..101
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 ataattttgt ttaactttaa gaaggagata tacatatgca ccatcatcat catcattctt
       61 ctggtgtaga tctgggtacc gagaacctgt acttccaatc catgctcttg tccaaaatca
      121 actcgcttgc ccacctgcgc gccgcgccct gcaacgacct gcacgccacc aagctggcgc
      181 ccggcaagga gaaggagccc ctggagtcgc agtaccaggt gggcccgcta ctgggcagcg
      241 gcggcttcgg ctcggtctac tcaggcatcc gcgtctccga caacttgccg gtggccatca
      301 aacacgtgga gaaggaccgg atttccgact ggggagagct gcctaatggc actcgagtgc
      361 ccatggaagt ggtcctgctg aagaaggtga gctcgggttt ctccggcgtc attaggctcc
      421 tggactggtt cgagaggccc gacagtttcg tcctgatcct ggagaggccc gagccggtgc
      481 aagatctctt cgacttcatc acggaaaggg gagccctgca agaggagctg gcccgcagct
      541 tcttctggca ggtgctggag gccgtgcggc actgccacaa ctgcggggtg ctccaccgcg
      601 acatcaagga cgaaaacatc cttatcgacc tcaatcgcgg cgagctcaag ctcatcgact
      661 tcgggtcggg ggcgctgctc aaggacaccg tctacacgga cttcgatggg acccgagtgt
      721 atagccctcc agagtggatc cgctaccatc gctaccatgg caggtcggcg gcagtctggt
      781 ccctggggat cctgctgtat gatatggtgt gtggagatat tcctttcgag catgacgaag
      841 agatc
//
