LOCUS       sequence-52442-e         848 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 848)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 848)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: AK1
FEATURES             Location/Qualifiers
     source          1..848
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(21..38)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     regulatory      444..453
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(689..709)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(734..751)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             762..784
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    799..823
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(823..842)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(824..842)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 cttgtagcag ccggatctca gtggtggtgg tggtggtgct cgagtgcggc cgcaagcttg
       61 tcgacggagc tcgaattcgg atccgtatcc acctttactg tcaattcaat agggcgtcca
      121 ggtgggtgca gacctgggag aagacactgt ccacggagcc ctcagcgttg accttgcgca
      181 caatgccacg tttctcatag aaggcgatga cgggttctgt ggccttgtaa taggtctcca
      241 gccgcttttt gatggtctcc tcattgtcgt ccacacgccc gctggtctct ccacgtttca
      301 agagccgctg ggtcatggtc tcagggcctg cgtccacata cagcagcagt gtgggctgtc
      361 caatccgtcg ctcaaactct tctccttgct gcacctcccg cgggtagcca tcaatcagga
      421 agcctttgga agtattgact ttggccacca tggcatcccg gagcatgtcc aacactgtct
      481 ccagtggaac cagctgcccc ttctccatga tttccgacag cttcttgccc ctggccgagc
      541 ctgagctgac ctcggaccgc aggaggtccc cggtggagag gtgggtgtag ccatacttct
      601 gcacgatctt ctcacactgg gtgcccttcc ctgagccagg cccacccacc acaaagatga
      661 tattggtttt cttcagcttc tcttccatgg attggaagta caggttctcg gtacccagat
      721 ctacaccaga agaatgatga tgatgatggt gcatatgtat atctccttct taaagttaaa
      781 caaaattatt tctagagggg aattgttatc cgctcacaat tcccctatag tgagtcgtat
      841 taatttcg
//
