LOCUS       sequence-52443-e         893 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 893)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 893)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: AK1
FEATURES             Location/Qualifiers
     source          1..893
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             13..35
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             46..63
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             88..108
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     regulatory      344..353
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             759..776
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(829..847)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
ORIGIN
        1 ccctctgaat attttgttta actttaagaa ggagatatac atatgcacca tcatcatcat
       61 cattcttctg gtgtagatct gggtaccgag aacctgtact tccaatccat ggaagagaag
      121 ctgaagaaaa ccaatatcat ctttgtggtg ggtgggcctg gctcagggaa gggcacccag
      181 tgtgagaaga tcgtgcagaa gtatggctac acccacctct ccaccgggga cctcctgcgg
      241 tccgaggtca gctcaggctc ggccaggggc aagaagctgt cggaaatcat ggagaagggg
      301 cagctggttc cactggagac agtgttggac atgctccggg atgccatggt ggccaaagtc
      361 aatacttcca aaggcttcct gattgatggc tacccgcggg aggtgcagca aggagaagag
      421 tttgagcgac ggattggaca gcccacactg ctgctgtatg tggacgcagg ccctgagacc
      481 atgacccagc ggctcttgaa acgtggagag accagcgggc gtgtggacga caatgaggag
      541 accatcaaaa agcggctgga gacctattac aaggccacag aacccgtcat cgccttctat
      601 gagaaacgtg gcattgtgcg caaggtcaac gctgagggct ccgtggacag tgtcttctcc
      661 caggtctgca cccacctgga cgccctattg aattgacagt aaaggtggat acggatccga
      721 attcgagctc cgtcgacaag cttgcggccg cactcgagca ccaccaccac caccactgag
      781 atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat
      841 aactagcata accccttggg gcctctaacg gtcttgaggg tttttgctga aga
//
