LOCUS       sequence-52444-e         909 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 909)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 909)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: AK3L1
FEATURES             Location/Qualifiers
     source          1..909
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(23..40)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(778..798)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(823..840)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ggcttgtagc agccggatct cagtggtggt ggtggtggtg ctcgagtgcg gccgcaagct
       61 tgtcgacgga gctcgaattc ggatccgtat ccacctttac tgtcacaaat atgcttcttt
      121 ggactgaata ggtgtgatct tgtttgagaa aagtgtgtaa acgtagggcc agattttgtt
      181 cgtctccgtt ccggaaaatt ggtggagcac tcctcggctc ttgtataatt caatgactgg
      241 ctttgccacg tctttgtact gtcttagcct ggcagcaact gcttcgggtt tatcatcctc
      301 ctgctggact aacggttcac cagtgacgtc atcaatacca tgtacatgag gtggattgaa
      361 gtccaggtta tatacccttc cgctaggagg gtgaatccaa cggcggctga gacgatcttt
      421 aagtgtttca aatggaatat tcaaactgat cactagatcc acttcacaga ttttgtccag
      481 ggcttcggct tgtcctaatg tcctaggaaa accatcaagg agccagtgct ggccacgcct
      541 gttctccaac tcggacatca ttaggcgtgt gatcacatgg tctggaacca aaagactttt
      601 ctctatatac tgctttgcca tctcaccaac ttcggtgctg gccttgatgt tctcccgcaa
      661 gaagtggccg ctggagagat gctggagacc aaagttctgg gcgatcctct ggcacacggt
      721 gcccttgccc gagccgggcg gcccgaggat gaccgcgcgc aggagtttgg aagccatgga
      781 ttggaagtac aggttctcgg tacccagatc tacaccagaa gaatgatgat gatgatggtg
      841 catatgtata tctccttctc ataaagttaa acaaattatt tctagaaggg gaaattgtta
      901 tccgctcac
//
