LOCUS       sequence-52445-e         874 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 874)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 874)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: AK3L1
FEATURES             Location/Qualifiers
     source          1..874
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             8..30
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             41..58
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             83..103
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             841..858
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tgaatatttt gtttaacttt aagaaggaga tatacatatg caccatcatc atcatcattc
       61 ttctggtgta gatctgggta ccgagaacct gtacttccaa tccatggctt ccaaactcct
      121 gcgcgcggtc atcctcgggc cgcccggctc gggcaagggc accgtgtgcc agaggatcgc
      181 ccagaacttt ggtctccagc atctctccag cggccacttc ttgcgggaga acatcaaggc
      241 cagcaccgaa gttggtgaga tggcaaagca gtatatagag aaaagtcttt tggttccaga
      301 ccatgtgatc acacgcctaa tgatgtccga gttggagaac aggcgtggcc agcactggct
      361 ccttgatggt tttcctagga cattaggaca agccgaagcc ctggacaaaa tctgtgaagt
      421 ggatctagtg atcagtttga atattccatt tgaaacactt aaagatcgtc tcagccgccg
      481 ttggattcac cctcctagcg gaagggtata taacctggac ttcaatccac ctcatgtaca
      541 tggtattgat gacgtcactg gtgaaccgtt agtccagcag gaggatgata aacccgaagc
      601 agttgctgcc aggctaagac agtacaaaga cgtggcaaag ccagtcattg aattatacaa
      661 gagccgagga gtgctccacc aattttccgg aacggagacg aacaaaatct ggccctacgt
      721 ttacacactt ttctcaaaca agatcacacc tattcagtcc aaagaagcat atttgtgaca
      781 gtaaaggtgg atacggatcc gaattcgagc tccgtcgaca agcttgcggc cgcactcgag
      841 caccaccacc accaccactg agatccggct gcta
//
