LOCUS       sequence-52449-e        1119 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1119)
  AUTHORS   Kowalczyk SW, Kapinos L, Blosser TR, Magalhaes T, van Nies P, Lim 
            RY, Dekker C
  TITLE     Single-molecule transport across an individual biomimetic nuclear 
            pore complex.
  JOURNAL   Nat Nanotechnol. 2011 Jun 19;6(7):433-8. doi: 10.1038/nnano.2011.88.
  PUBMED    21685911
REFERENCE   2  (bases 1 to 1119)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nnano.2011"; journalName: "Nat Nanotechnol"; date: 
            "2011-06-19- 19"; volume: "6"; issue: "7"; pages: "433-8"
COMMENT     Sequence Label: pPEP-TEV cNup98 (1-498)
FEATURES             Location/Qualifiers
     source          1..1119
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             41..58
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             179..199
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 annttttgtt tactttaaga anggagatat accannnggc caccatcacc atcatcacat
       61 gcaaaaatac ttagaagata aagctcaaga attggtgcga ctggaaggag aggttcgctc
      121 cctccttaag gacataagtg agaaagttgc ggtttacagc accggcggtg gtggcggtga
      181 gaatttatat tttcaagggg gatcctgttg ctgttttaac aaatcatttg gaacaccctt
      241 tgggggtggc acaggtggct ttggcacaac ttcaacattt ggacagaata ctggctttgg
      301 cactactagt ggaggggcat ttggaacatc tgcatttggt tctagcaaca atactggagg
      361 cctctttgga aattcacaga ctaaaccagg aggattgttt ggaaccagtt catttagcca
      421 gccagctacc tccacaagca ctggctttgg gtttggtacg tcaacaggaa cagcaaatac
      481 cttgtttgga actgcaagca cagggaccag tctcttctca tcccaaaaca atgcctttgc
      541 acaaaataaa ccaactggct ttggcaattt tggaaccagt actagcagtg gaggactctt
      601 tggaaccaca aataccacct ctaatccttt tggcagcaca tctggctccc tctttgggcc
      661 aagtagtttt acagctgctc ctactgggac tactattaaa tttaaccctc caactggtac
      721 agatactatg gtcaaagctg gagttagcac taacataagt accaagcacc agtgtattac
      781 tgctatgana gaatatgaaa gcaagtcact agaggaactt cgtttagagg attatcaggc
      841 taacaggaag ggcccacaga accaggtggg agcaggtacc acaactggct tgtttgggtc
      901 ttctccagcc acttccagcg caacaggact cttcagctcc tccaccacta attcaggctt
      961 tgcatatggt cagaacaaaa ctgcctttgg aactagtaca actggatttg gaacanatcc
     1021 aggtggtctc tttggccaac agaatcagca gactaccagc ctcttcagca aaccatttgg
     1081 ccaggntaca accncccana annctggctt ttcctttgg
//
