LOCUS       sequence-52462-e         872 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 872)
  AUTHORS   Silvers R, Saxena K, Kudlinzki D, Schwalbe H
  TITLE     Recombinant expression and purification of human TATA binding 
            protein using a chimeric fusion.
  JOURNAL   Protein Expr Purif. 2012 Jul 24;85(1):142-147.
  PUBMED    22841618
REFERENCE   2  (bases 1 to 872)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr Purif."; date: "2012-07-24"; volume: "85(1)"; pages: "142-147"
COMMENT     Sequence Label: pRS-45
FEATURES             Location/Qualifiers
     source          1..872
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             15..32
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             42..59
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             294..314
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 ccatgggcag ctctcatcac catcaccatc acagtagcgg tctggtgccg cgtggtagcc
       61 acatgggcaa aaccaacctg gccaatgaag atgaagcgta tgaagccatt tttggcggtg
      121 aattcggttc tctggaaatt ggcagttata tcggcggtga tgaaggcgcg aactctaaag
      181 attacacgga acacctgccg gatgcagttg attttgaaga tgaagatgaa ctggcggatg
      241 atgatgatga tctgccgggc ggtggctctg gtggcggtag tggcggtggc agcgagaatc
      301 tttattttca gggccatatg agcggcattg ttccgcagct gcagaatatt gtgagcaccg
      361 ttaatctggg ttgcaaactg gatctgaaaa ccattgccct gcgcgcccgc aacgcggaat
      421 ataacccgaa acgcttcgcg gcggttatta tgcgcattcg cgaaccgcgt accaccgcgc
      481 tgatttttag cagcggtaaa atggtgtgca ccggcgccaa aagcgaagaa cagagccgtc
      541 tggcggcccg taaatatgcg cgcgttgttc agaaactggg ctttccggcg aaatttctgg
      601 attttaaaat tcagaacatg gtgggcagct gcgatgttaa atttccgatt cgtctggaag
      661 gcctggttct gacccatcag cagtttagca gctatgaacc ggaactgttt ccgggtctga
      721 tttatcgtat gatcaaaccg cgtattgtgc tgctgatttt tgtgagcggc aaagttgtgc
      781 tgaccggtgc gaaagttcgt gcggaaattt atgaagcgtt tgaaaacatt tatccgatcc
      841 tgaaaggttt tcgcaaaacc acctaaggat cc
//
