LOCUS       sequence-52470-e         956 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 956)
  TITLE     Chin Lab P85 plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 956)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLenti4/V5-DEST-P85(DKRMNS560del)
FEATURES             Location/Qualifiers
     source          1..956
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(7..48)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    101..125
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ggtacgcgta gaatcgagac cgaggagagg gttagggata ggcttacctt cgaaccgcgg
       61 gccctctaga ctcgagcggc cgccactgtg ctggatatca accactttgt acaagaaagc
      121 tgggtcggcg cgcccaccct taattaatca tcgcctctgc tgtgcatata ctgggtaggc
      181 tagtgtgaca ttgagggagt cgttgtgctg cacaagggag gtgtgttggt aatgtagcac
      241 cagttctttc agagagctgt acaagttata gggctcggca aagccatagc cagttgctgt
      301 tttgtttatg acacaatgct ttacttcgcc gtccaccact acagagcagg catagcagcc
      361 ctgtttactg ctctcccgga caagaaaagt gccatctcgc ttccctcgca acaggttttc
      421 agctttgttt cggttgctgc ttccaacatt ccatgtcttc tcatcatgat ggggcaaatc
      481 ttcatcatct tccaccagtg aatattggtc ttcagtgttt tcattgccca accactcgtt
      541 caacttcttt tgccgaacac ctttttgagt caaccacatc aagtattggt ctctcgtctt
      601 tctcagctgg ataaggtctg gtttaatgat ttctcgatac tcagctgcct gcttcttcaa
      661 gtcttcttcc aatcttcttc tactgtcaat aatttcactg attcgagact tcaacttatc
      721 ataattatgc ataatccttt gtatttcttt ctcattgcct tcacgtttaa acttttctat
      781 gtattctttg ctgtaccgct cttgggtctg gcactgttct tcaaatattt ttatggtttc
      841 attaaatgct tcaatagctg tccttttcat ttggatttcc tgggatgtgc gggtatattc
      901 ttcatataat ctatcatatt ctcgactttt ttcttgaaac tgagtgttat attcat
//
