LOCUS       sequence-52473-e         887 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 887)
  TITLE     Chin Lab P85 plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 887)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLenti4/V5-DEST-P85(T576del)
FEATURES             Location/Qualifiers
     source          1..887
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(7..48)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    101..125
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ggtacgcgta gaatcgagac cgaggagagg gttagggata ggcttacctt cgaaccgcgg
       61 gccctctaga ctcgagcggc cgccactgtg ctggatatca accactttgt acaagaaagc
      121 tgggtcggcg cgcccaccct taattaatca tcgcctctgc tgtgcatata ctgggtaggc
      181 tagtgtgaca ttgagggagt cgttgtgctg cacaagggag gtgtgttggt aatgtagcac
      241 cagttctttc agagagctgt acaagttata gggctcggca aagccatagc cagttgctgt
      301 tttgtttatg acacaatgct ttacttcgcc gtccaccact acagagcagg catagcagcc
      361 ctgtttactg ctctcccgga caagaaaagt gccatctcgc ttccctcgca acaggttttc
      421 agctttgttt cggttgctgc ttccaacatt ccatgtcttc tcatcatgat ggggcaaatc
      481 ttcatcatct tccaccagtg aatattggtc ttcagtgttt tcattgccca accactcgtt
      541 caacttcttt tgccgaacac ctttttgagt caaccacatc aagtattggt ctctctttct
      601 cagctggata aggtctggtt taatgctgtt catacgtttg tcaatttctc gatactcagc
      661 tgcctgcttc ttcaagtctt cttccaatct tcttctactg tcaataattt cactgattcg
      721 agacttcaac ttatcataat tatgcataat cctttgtatt tctttctcat tgccttcacg
      781 tttaaacttt tctatgtatt ctttgctgta ccgctcttgg gtctggcact gttcttcaaa
      841 tatttttatg gtttcattaa atgcttcaat agctgtcctt ttcattt
//
