LOCUS       sequence-52474-e         926 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 926)
  AUTHORS   Goldstein AL, McCusker JH
  TITLE     Three new dominant drug resistance cassettes for gene disruption in 
            Saccharomyces cerevisiae.
  JOURNAL   Yeast. 1999 Oct;15(14):1541-53.
  PUBMED    10514571
REFERENCE   2  (bases 1 to 926)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast."; 
            date: "1999-10"; volume: "15(14)"; pages: "1541-53"
COMMENT     Sequence Label: pAG26
FEATURES             Location/Qualifiers
     source          1..926
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      145..342
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     primer_bind     complement(458..477)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(459..477)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(484..501)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(484..500)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(493..515)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(728..747)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 cgtccgatcc ggagccggga ctgtcgggcg tacacaaatc gcccgcagaa gcgcggccgt
       61 ctggaccgat ggctgtgtag aagtactcgc cgatagtgga aaccgacgcc ccagcactcg
      121 tccgagggca aaggaataat cagtactgac aataaaaaga ttcttgtttt caagaacttg
      181 tcatttgtat agttttttta tattgtagtt gttctatttt aatcaaatgt tagcgtgatt
      241 tatatttttt ttcgcctcga catcatctgc ccagatgcga agttaagtgc gcagaaagta
      301 atatcatgcg tcaatcgtat gtgaatgctg gtcgctatac tgctgtcgat tcgatactaa
      361 cgccgccatc cagtgtcgaa aacgagctcg aattcatcga tgatatcaga tccactagtg
      421 gcctatgcgg ccgccaccgc ggtggagctc caattcgccc tatagtgagt cgtattacaa
      481 ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa
      541 tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga
      601 tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg acgcgccctg tagcggcgca
      661 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
      721 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
      781 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
      841 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
      901 tttcgccctt tgacgttgga gtccac
//
