LOCUS       sequence-52476-e         824 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 824)
  AUTHORS   Ito-Harashima S, McCusker JH
  TITLE     Positive and negative selection LYS5MX gene replacement cassettes 
            for use in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2004 Jan 15;21(1):53-61.
  PUBMED    14745782
REFERENCE   2  (bases 1 to 824)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast."; 
            date: "2004-01-15"; volume: "21(1)"; pages: "53-61"
COMMENT     Sequence Label: pSA25
FEATURES             Location/Qualifiers
     source          1..824
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(130..327)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
ORIGIN
        1 gactcactat agggcgaatt ggagctccac cgcggtggcg gccgcatagg ccactagtgg
       61 atctgatatc atcgatgaat tcgagctcgt tttcgacact ggatggcggc gttagtatcg
      121 aatcgacagc agtatagcga ccagcattca catacgattg acgcatgata ttactttctg
      181 cgcacttaac ttcgcatctg ggcagatgat gtcgaggcga aaaaaaatat aaatcacgct
      241 aacatttgat taaaatagaa caactacaat ataaaaaaac tatacaaatg acaagttctt
      301 gaaaacaaga atctttttat tgtcagtact gattataaac catcattttc gatgaaataa
      361 tcaatcaacg tagataagct gatattatat aattttggtc tgttcgtgtt gattttatca
      421 ctgatggact ttggcataca gatagtgaca atttcgttat tgaaccattg agaatggaaa
      481 atcaatggaa cttcatccag agttatgcac atagaagctc cctcagccgg aaaaaagctg
      541 atagcgccaa aatctattag tgacaagtct gtgttaaggc cagttccagt aaattttgta
      601 tacgactcct tcaaggacca taagtaagta aatattgtgc atggatcaga cgctttcagt
      661 aaaccgttaa attctctttc actaaaaact tctttaaata gctccaactc ttccctcccg
      721 ccataattgc acggagaagc gatatcaatt ccgacatcct ggtattcatc tgtacttaca
      781 cattttacga ggaacatagc tacatattgt tcaccgatgg tcat
//
