LOCUS       sequence-52478-e         838 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 838)
  AUTHORS   Sinha H, Nicholson BP, Steinmetz LM, McCusker JH
  TITLE     Complex genetic interactions in a quantitative trait locus.
  JOURNAL   PLoS Genet. 2006 Feb;2(2):e13. Epub 2006 Feb 3.
  PUBMED    16462944
REFERENCE   2  (bases 1 to 838)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Genet."; date: "2006-02"; volume: "2(2)"; pages: "e13. Epub 2006 Feb
            3"
COMMENT     Sequence Label: pBN49
FEATURES             Location/Qualifiers
     source          1..838
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      148..345
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     primer_bind     complement(452..471)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(453..471)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(558..575)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(809..828)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 catcgtccga tccggagccg ggactgtcgg gcgtacacaa atcgcccgca gaagcgcggc
       61 cgtctggacc gatggctgtg tagaagtact cgccgatagt ggaaaccgac gccccagcac
      121 tcgtccgagg gcaaaggaat aatcagtact gacaataaaa agattcttgt tttcaagaac
      181 ttgtcatttg tatagttttt ttatattgta gttgttctat tttaatcaaa tgttagcgtg
      241 atttatattt tttttcgcct cgacatcatc tgcccagatg cgaagttaag tgcgcagaaa
      301 gtaatatcat gcgtcaatcg tatgtgaatg ctggtcgcta tactgctgtc gattcgatac
      361 taacgccgcc atccagtgtc gaaaacgagc tcgaattcat cgatgatatc agatccacta
      421 gtggcctatg cggccgcgga tctgccggtc tccctatagt gagtcgtatt aatttcgata
      481 agccaggtta acctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
      541 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
      601 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
      661 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
      721 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
      781 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagct
//
