LOCUS       sequence-52481-e        2520 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2520)
  AUTHORS   Azarin SM, Lian X, Larson EA, Popelka HM, de Pablo JJ, Palecek SP
  TITLE     Modulation of Wnt/beta-catenin signaling in human embryonic stem 
            cells using a 3-D microwell array.
  JOURNAL   Biomaterials. 2012 Mar;33(7):2041-9. Epub 2011 Dec 15.
  PUBMED    22177620
REFERENCE   2  (bases 1 to 2520)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biomaterials."; date: "2012-03"; volume: "33(7)"; pages: "2041-9. 
            Epub 2011 Dec 15"
COMMENT     Sequence Label: pXL010-Wnt dual (GFP-Fire) reporter
FEATURES             Location/Qualifiers
     source          1..2520
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..666
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina 
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /label=CopGFP
                     /translation="MPAMKIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGAL
                     TFSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYR
                     YEAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDG
                     GYYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA
                     "
     CDS             1..666
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina 
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /label=CopGFP
                     /translation="MPAMKIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGAL
                     TFSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYR
                     YEAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDG
                     GYYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA
                     "
     CDS             808..861
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             868..2520
                     /codon_start=1
                     /gene="luc"
                     /product="firefly luciferase"
                     /label=luciferase
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     primer_bind     complement(934..952)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
ORIGIN
        1 atgcccgcca tgaagatcga gtgccgcatc accggcaccc tgaacggcgt ggagttcgag
       61 ctggtgggcg gcggagaggg cacccccgag cagggccgca tgaccaacaa gatgaagagc
      121 accaaaggcg ccctgacctt cagcccctac ctgctgagcc acgtgatggg ctacggcttc
      181 taccacttcg gcacctaccc cagcggctac gagaacccct tcctgcacgc catcaacaac
      241 ggcggctaca ccaacacccg catcgagaag tacgaggacg gcggcgtgct gcacgtgagc
      301 ttcagctacc gctacgaggc cggccgcgtg atcggcgact tcaaggtggt gggcaccggc
      361 ttccccgagg acagcgtgat cttcaccgac aagatcatcc gcagcaacgc caccgtggag
      421 cacctgcacc ccatgggcga taacgtgctg gtgggcagct tcgcccgcac cttcagcctg
      481 cgcgacggcg gctactacag cttcgtggtg gacagccaca tgcacttcaa gagcgccatc
      541 caccccagca tcctgcagaa cgggggcccc atgttcgcct tccgccgcgt ggaggagctg
      601 cacagcaaca ccgagctggg catcgtggag taccagcacg ccttcaagac ccccatcgcc
      661 ttcgccagat ctcgagatat cagccatggc ttcccgccgg cggtggcggc gcaggatgat
      721 ggcacgctgc ccatgtcttg tgcccaggag agcgggatgg accgtcaccc tgcagcctgt
      781 gcttctgcta ggatcaatgt gaccggtgag ggcagaggaa gtcttctaac atgcggtgac
      841 gtggaggaga atcccggccc ttccggtatg gaagacgcca aaaacataaa gaaaggcccg
      901 gcgccattct atccgctaga ggatggaacc gctggagagc aactgcataa ggctatgaag
      961 agatacgccc tggttcctgg aacaattgct tttacagatg cacatatcga ggtgaacatc
     1021 acgtacgcgg aatacttcga aatgtccgtt cggttggcag aagctatgaa acgatatggg
     1081 ctgaatacaa atcacagaat cgtcgtatgc agtgaaaact ctcttcaatt ctttatgccg
     1141 gtgttgggcg cgttatttat cggagttgca gttgcgcccg cgaacgacat ttataatgaa
     1201 cgtgaattgc tcaacagtat gaacatttcg cagcctaccg tagtgtttgt ttccaaaaag
     1261 gggttgcaaa aaattttgaa cgtgcaaaaa aaattaccaa taatccagaa aattattatc
     1321 atggattcta aaacggatta ccagggattt cagtcgatgt acacgttcgt cacatctcat
     1381 ctacctcccg gttttaatga atacgatttt gtaccagagt cctttgatcg tgacaaaaca
     1441 attgcactga taatgaactc ctctggatct actgggttac ctaagggtgt ggcccttccg
     1501 catagaactg cctgcgtcag attctcgcat gccagagatc ctatttttgg caatcaaatc
     1561 attccggata ctgcgatttt aagtgttgtt ccattccatc acggttttgg aatgtttact
     1621 acactcggat atttgatatg tggatttcga gtcgtcttaa tgtatagatt tgaagaagag
     1681 ctgtttttac gatcccttca ggattacaaa attcaaagtg cgttgctagt accaacccta
     1741 ttttcattct tcgccaaaag cactctgatt gacaaatacg atttatctaa tttacacgaa
     1801 attgcttctg ggggcgcacc tctttcgaaa gaagtcgggg aagcggttgc aaaacgcttc
     1861 catcttccag ggatacgaca aggatatggg ctcactgaga ctacatcagc tattctgatt
     1921 acacccgagg gggatgataa accgggcgcg gtcggtaaag ttgttccatt ttttgaagcg
     1981 aaggttgtgg atctggatac cgggaaaacg ctgggcgtta atcagagagg cgaattatgt
     2041 gtcagaggac ctatgattat gtccggttat gtaaacaatc cggaagcgac caacgccttg
     2101 attgacaagg atggatggct acattctgga gacatagctt actgggacga agacgaacac
     2161 ttcttcatag ttgaccgctt gaagtcttta attaaataca aaggatacca ggtggccccc
     2221 gctgaattgg agtcgatatt gttacaacac cccaacatct tcgacgcggg cgtggcaggt
     2281 cttcccgacg atgacgccgg tgaacttccc gccgccgttg ttgttttgga gcacggaaag
     2341 acgatgacgg aaaaagagat cgtggattac gtcgccagtc aagtaacaac cgcgaaaaag
     2401 ttgcgcggag gagttgtgtt tgtggacgaa gtaccgaaag gtcttaccgg aaaactcgac
     2461 gcaagaaaaa tcagagagat cctcataaag gccaagaagg gcggaaagtc caaattgtaa
//
