LOCUS       sequence-52484-e         929 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 929)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 929)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: RGS10
FEATURES             Location/Qualifiers
     source          1..929
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(23..40)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(517..537)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(562..579)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             590..612
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    627..651
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(651..670)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(652..670)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(758..777)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 gcttggtagc agccggatct cagtggtggt ggtggtggtg ctcgagtgcg gccgcaagct
       61 tgtcgacgga gctcgaattc ggatccgtat ccacctttac tgtcacaaat cttcttcctc
      121 ttcctcggtt cgcttgtgtt ttaaaaacaa gtcagacttt aagaagcggc tgtagctgtc
      181 gtacttcatg agattaaaga tctggtcctg gagtttctgg aacatcagag ggtgcggttc
      241 ttccaggatc ttctcgttga gccgagactg cccctccacg ttgacctgtg atgaggcctt
      301 gctggacaga aaggtcatgt agatctcctt tgccttttcc tgcatctgcg tcttatcttg
      361 cattttctta aaatcttcac atgctagcca aaacaaaaca ttttcttcac tgaattcctt
      421 ttttaaaaat tccctaaatc ttttcacgcc ttctgggtct tccagcagat tctccaggga
      481 tgccgcccat ttggctgtgc tcttgaggct ctgcatggat tggaagtaca ggttctcggt
      541 acccagatct acaccagaag aatgatgatg atgatggtgc atatgtatat ctccttctta
      601 aagttaaaca aaattatttc tagaggggaa ttgttatccg ctcacaattc ccctatagtg
      661 agtcgtatta atttcgcggg atcgagatcg atctcgatcc tctacgccgg acgcatcgtg
      721 gccggcatca ccggcgccac aggtgcggtt gctggcgcct atatcgccga catcaccgat
      781 ggggaagatc gggctcgcca cttcgggctc atgagcgctt gtttcggcgt gggtatggtg
      841 gcaggccccg tggccggggg actgttgggc gccatctcct tgcatgcacc attcccttgc
      901 ggcgggcggt gctcaacggg cctcaacct
//
