LOCUS       sequence-52485-e         899 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 899)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 899)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: RGS10
FEATURES             Location/Qualifiers
     source          1..899
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             43..60
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             85..105
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             582..599
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(652..670)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      666..713
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(837..856)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ctctgaatat tttgtttact ttaagaagga gatatacata tgcaccatca tcatcatcat
       61 tcttctggtg tagatctggg taccgagaac ctgtacttcc aatccatgca gagcctcaag
      121 agcacagcca aatgggcggc atccctggag aatctgctgg aagacccaga aggcgtgaaa
      181 agatttaggg aatttttaaa aaaggaattc agtgaagaaa atgttttgtt ttggctagca
      241 tgtgaagatt ttaagaaaat gcaagataag acgcagatgc aggaaaaggc aaaggagatc
      301 tacatgacct ttctgtccag caaggcctca tcacaggtca acgtggaggg gcagtctcgg
      361 ctcaacgaga agatcctgga agaaccgcac cctctgatgt tccagaaact ccaggaccag
      421 atctttaatc tcatgaagta cgacagctac agccgcttct taaagtctga cttgttttta
      481 aaacacaagc gaaccgagga agaggaagaa gatttgtgac agtaaaggtg gatacggatc
      541 cgaattcgag ctccgtcgac aagcttgcgg ccgcactcga gcaccaccac caccaccact
      601 gagatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc
      661 aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag
      721 gaggaactat atccggattg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc
      781 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
      841 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaa
//
