LOCUS       sequence-52486-e         923 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 923)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 923)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: RGS14
FEATURES             Location/Qualifiers
     source          1..923
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(15..32)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(557..577)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(602..619)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             630..652
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    667..691
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(691..710)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(692..710)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(798..817)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 cagccggaat ctcagtggtg gtggtggtgg tgctcgagtg cggccgcaag cttgtcgacg
       61 gagctcgaat tcggatccgt atccaccttt actgtcacgt ggcgtcaggg ctgccgaggc
      121 gcgaggagcc aggttcccgc agagggcgtc cctcggcttc ggctagcagg cactcgcggt
      181 acagcgggga cttgacgaag cgcgcatagc tgtcgaactt catcaagttg aagatctgaa
      241 gctgctgtgc ccgaaacatg tccggccggg gctcggccag cacctcctcg ccaagccagg
      301 cctgacggtc gatgttcact gggctcagcg cctggctgga caggaactcc tggtagatgt
      361 tgcgggcctc ctgagctagc tgctgggtat cgctggccgg gatctgctgg aagcgctcgc
      421 aggccttcca gaaagtcacg ttttccgcgc tgaactcctt cttcaggaac tcagtgaagt
      481 aagccaggcc cagcgggtcc tgcaacagcc gctcgaagga cagggcccag ctggccacag
      541 gctgctcctc ggtcatggat tggaagtaca ggttctcggt acccagatct acaccagaag
      601 aatgatgatg atgatggtgc atatgtatat ctccttctta aagttaaaca aaattatttc
      661 tagaggggaa ttgttatccg ctcacaattc ccctatagtg agtcgtatta atttcgcggg
      721 atcgagatcg atctcgatcc tctacgccgg acgcatcgtg gccggcatca ccggcgccac
      781 aggtgcggtt gctggcgcct atatcgccga catcaccgat ggggaagatc gggctcgcca
      841 cttcgggctc atgagcgctt gttttccgcg tgggtatggt ggcaggcccg tggccggggg
      901 actgttggcg cccattctcc ttg
//
