LOCUS       sequence-52487-e         611 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry vector for shRNA expression under H1/TO promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 611)
  AUTHORS   Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, 
            Campisi J, Yaswen P, Cooper PK, Kaufman PD.
  TITLE     A versatile viral system for expression and depletion of proteins in
            mammalian cells.
  JOURNAL   PLoS One. 2009 Aug 6;4(8):e6529.
  PUBMED    19657394
REFERENCE   2  (bases 1 to 611)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2009-08-6"; volume: "4(8)"; pages: "e6529"
COMMENT     Sequence Label: pENTR/pTER+ (430-1)
FEATURES             Location/Qualifiers
     source          1..611
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    12..30
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 atcttataag ttccctatca gtgatagaga tctcggcgtt gtgcatgtcg ggcgactgct
       61 ccatgatctt gcgccgctcg atctgcgacc acaccataaa ggcgttcatg ggccgcttga
      121 tgtggccact gggcgtcttg caccagctgg ggtcgtccgc cttgccgccc gtcgacgcgg
      181 tggagccagg cgtcggggag gacgcgatgc ccagctccag gccggcgccc gagtccgagc
      241 tctccccggc cagcagagcc tcagtgttct ccgcgttgtt ggtctgttgt accatggccc
      301 cggcttgccg ggcgcccacg cgcgctcaca cgctcgcggc gcggtcgccg ggccctgcgg
      361 gctccccggg gcggagacca gggtcccctg agcggcgcgc gggccagggg cggccgcctc
      421 ccgccgccaa cctagagtcc ccgaggccct ccgtcctccc tcaccgctga agtgctccct
      481 gcgggcaccg aggctggagt cggccgcggc gaggagtttc tccaaacgtt ggtagccgga
      541 gtatcttcca aacgcaagtt tctcgccgcc tccggggcaa gtctttcccc cacccctttc
      601 cctgaagcag t
//
