LOCUS       sequence-52488-e         870 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 870)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 870)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: RGS14
FEATURES             Location/Qualifiers
     source          1..870
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             11..33
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             44..61
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             86..106
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             631..648
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(701..719)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      715..762
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 tctagaatat tttgtttaac tttaagaagg agatatacat atgcaccatc atcatcatca
       61 ttcttctggt gtagatctgg gtaccgagaa cctgtacttc caatccatga ccgaggagca
      121 gcctgtggcc agctgggccc tgtccttcga gcggctgttg caggacccgc tgggcctggc
      181 ttacttcact gagttcctga agaaggagtt cagcgcggaa aacgtgactt tctggaaggc
      241 ctgcgagcgc ttccagcaga tcccggccag cgatacccag cagctagctc aggaggcccg
      301 caacatctac caggagttcc tgtccagcca ggcgctgagc ccagtgaaca tcgaccgtca
      361 ggcctggctt ggcgaggagg tgctggccga gccccggccg gacatgtttc gggcacagca
      421 gcttcagatc ttcaacttga tgaagttcga cagctatgcg cgcttcgtca agtccccgct
      481 gtaccgcgag tgcctgctag ccgaagccga gggacgccct ctgcgggaac ctggctcctc
      541 gcgcctcggc agccctgacg ccacgtgaca gtaaaggtgg atacggatcc gaattcgagc
      601 tccgtcgaca agcttgcggc cgcactcgag caccaccacc accaccactg agatccggct
      661 gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca
      721 taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata
      781 tccggattgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
      841 ttacgcgcag cgtgaccgct acacttgcca
//
