LOCUS       sequence-52489-e         890 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 890)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 890)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: RGS16
FEATURES             Location/Qualifiers
     source          1..890
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(24..41)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(524..544)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(569..586)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             597..619
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    634..658
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(658..677)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(659..677)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(765..784)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 gggcttgtag cagccggatc tcagtggtgg tggtggtggt gctcgagtgc ggccgcaagc
       61 ttgtcgacgg agctcgaatt cggatccgta tccaccttta ctgtcaagtg gcagaggcgg
      121 ctgaggcttg ggcagccagg tcccggtaag caggcgactt caggaagcgt gggtaggagt
      181 ccttctccat cagggtacgt gtcttcccct gagccgcatc aaagcatgtg gctgtggcag
      241 tctgcaggtt catcctcgtc agctcgcggg tctcatggtc aatgttgacc tctttagggg
      301 cctcactgca aatgaactcc tcaaagatct ggtgtgccct ggaggccagc ttggtagctg
      361 atcggatctt cttgaactcc tcacaggcca gccagaactc caggttctcc tcactgaact
      421 ctgtcttcag gaaagcgtgg aaggcagcca ctccattttt actgctcagc agcaggtcga
      481 acgactctct ccaccccagc acatcttctg agaagtttct catggattgg aagtacaggt
      541 tctcggtacc cagatctaca ccagaagaat gatgatgatg atggtgcata tgtatatctc
      601 cttcttaaag ttaaacaaaa ttatttctag aggggaattg ttatccgctc acaattcccc
      661 tatagtgagt cgtattaatt tcgcgggatc gagatcgatc tcgatcctct acgccggacg
      721 catcgtggcc ggcatcaccg gcgccacagg tgcggttgct ggcgcctata tcgccgacat
      781 caccgatggg gaagatcggg ctcgccactt cgggctcatg agcgcttgtt tcggcgtggg
      841 tatggtggca ggccccgtgg ccgggggact gttgggcgcc atctccttgc
//
