LOCUS       sequence-52490-e         870 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 870)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 870)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: RGS16
FEATURES             Location/Qualifiers
     source          1..870
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             42..59
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             84..104
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             587..604
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(657..675)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      671..718
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(842..861)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 tctgaatatt ttgtttactt taagaaggag atatacatat gcaccatcat catcatcatt
       61 cttctggtgt agatctgggt accgagaacc tgtacttcca atccatgaga aacttctcag
      121 aagatgtgct ggggtggaga gagtcgttcg acctgctgct gagcagtaaa aatggagtgg
      181 ctgccttcca cgctttcctg aagacagagt tcagtgagga gaacctggag ttctggctgg
      241 cctgtgagga gttcaagaag atccgatcag ctaccaagct ggcctccagg gcacaccaga
      301 tctttgagga gttcatttgc agtgaggccc ctaaagaggt caacattgac catgagaccc
      361 gcgagctgac gaggatgaac ctgcagactg ccacagccac atgctttgat gcggctcagg
      421 ggaagacacg taccctgatg gagaaggact cctacccacg cttcctgaag tcgcctgctt
      481 accgggacct ggctgcccaa gcctcagccg cctctgccac ttgacagtaa aggtggatac
      541 ggatccgaat tcgagctccg tcgacaagct tgcggccgca ctcgagcacc accaccacca
      601 ccactgagat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc
      661 tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct
      721 gaaaggagga actatatccg gattggcgaa tgggacgcgc cctgtagcgg cgcattaagc
      781 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
      841 gctcctttcg ctttcttccc ttcctttctc
//
