LOCUS       sequence-52491-e         894 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 894)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 894)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: RGS17
FEATURES             Location/Qualifiers
     source          1..894
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(23..40)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(514..534)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(559..576)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             587..609
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    624..648
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(648..667)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(649..667)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(755..774)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 cttggttagc agccggatct cagtggtggt ggtggtggtg ctcgagtgcg gccgcaagct
       61 tgtcgacgga gctcgaattc ggatccgtat ccacctttac tgtcaagagc cagcagtact
      121 ttcaacaaat gacttataaa tttgagagtt caaaaacctt ggaaaagaat ctctgtgcat
      181 taaagtatat atctgaagtt gggcatcttc atacatgtga ggattgggat ccaacagatt
      241 tctattgatc acctctctaa ctcgagaatc aagactgacc tcttttggtg atagtataga
      301 aatgtaatct tcatatatca tcctagcctt ttcttcaatt acttttttgt tctgctcctt
      361 ctttaagtct tcacaagcaa gccagaaaag taggttctct tcactgtatt ctgttcggag
      421 gaactctctg aaaaggtttc ttcctgctgg ggccttcatc atcttgtcaa aattttgaga
      481 ccaggacaag acttcctctg cagtggggtt catggattgg aagtacaggt tctcggtacc
      541 cagatctaca ccagaagaat gatgatgatg atggtgcata tgtatatctc cttcttaaag
      601 ttaaacaaaa ttatttctag aggggaattg ttatccgctc acaattcccc tatagtgagt
      661 cgtattaatt tcgcgggatc gagatcgatc tcgatcctct acgccggacg catcgtggcc
      721 ggcatcaccg gcgccacagg tgcggttgct ggcgcctata tcgccgacat caccgatggg
      781 gaagatcggg ctcgccactt cgggctcatg agcgcttgtt tcggcgtggg tatggtggca
      841 ggccccgtgg ccggggggac tgttggggcg ccatctcctt gcatgcacca tttc
//
