LOCUS       sequence-52492-e         850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: RGS17
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             42..59
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             84..104
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             578..595
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(648..666)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      662..709
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 tctgaatatt ttgtttactt taagaaggag atatacatat gcaccatcat catcatcatt
       61 cttctggtgt agatctgggt accgagaacc tgtacttcca atccatgaac cccactgcag
      121 aggaagtctt gtcctggtct caaaattttg acaagatgat gaaggcccca gcaggaagaa
      181 accttttcag agagttcctc cgaacagaat acagtgaaga gaacctactt ttctggcttg
      241 cttgtgaaga cttaaagaag gagcagaaca aaaaagtaat tgaagaaaag gctaggatga
      301 tatatgaaga ttacatttct atactatcac caaaagaggt cagtcttgat tctcgagtta
      361 gagaggtgat caatagaaat ctgttggatc ccaatcctca catgtatgaa gatgcccaac
      421 ttcagatata tactttaatg cacagagatt cttttccaag gtttttgaac tctcaaattt
      481 ataagtcatt tgttgaaagt actgctggct cttgacagta aaggtggata cggatccgaa
      541 ttcgagctcc gtcgacaagc ttgcggccgc actcgagcac caccaccacc accactgaga
      601 tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata
      661 actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg
      721 aactatatcc ggattggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt
      781 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
      841 gctttcttcc
//
