LOCUS       sequence-52494-e         837 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 837)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 837)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: RGS18
FEATURES             Location/Qualifiers
     source          1..837
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(21..38)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(554..574)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(599..616)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             627..649
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    664..688
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(688..707)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(689..707)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(795..814)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 cttgtagcag ccggatctca gtggtggtgg tggtggtgct cgagtgcggc cgcaagcttg
       61 tcgacggagc tcgaattcgg atccgtatcc acctttactg tcattcattg caggtaaatg
      121 agcgtgatcg tctcctaaga tttgttggtc tctgaggtct tccttccatc aagtctaaat
      181 agatgtcaga tttcagaaaa cgtgtataac tgtcttgttc catgagctga tacactctgc
      241 tttgtgcagc atcaaaactg tggagggtag gttgagtgat gctgtttgta atgacttctt
      301 ttgtgtgaaa atcaaggtta acctcttttg gggcatcagt ctgtataaat ttctcatata
      361 ttgcttttgc tttaaggtga atttgttgag gtcccttgct tttcttgaaa tcttcacagg
      421 ctatccaaaa ttcaatattt tcttcactga attcagtttt aagaaatctg gtaaaagcct
      481 ctagtccatc tctatgggaa agcagtttgt caaatgattc accccatttc actgcctctt
      541 caggggagac catggattgg aagtacaggt tctcggtacc cagatctaca ccagaagaat
      601 gatgatgatg atggtgcata tgtatatctc cttcttaaag ttaaacaaaa ttatttctag
      661 aggggaattg ttatccgctc acaattcccc tatagtgagt cgtattaatt tcgcgggatc
      721 gagatcgatc tcgatcctct acgccggacg catcgtggcc ggcatcaccg gcgccacagg
      781 tgcggttgct ggcgcctata tcgccgacat caccgatggg gaagatcggg ctcgcca
//
