LOCUS       sequence-52495-e         904 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 904)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 904)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: RGS18
FEATURES             Location/Qualifiers
     source          1..904
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             48..65
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             90..110
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             626..643
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(696..714)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      710..757
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 attccctctg aatattttgt ttactttaag aaggagatat acatatgcac catcatcatc
       61 atcattcttc tggtgtagat ctgggtaccg agaacctgta cttccaatcc atggtctccc
      121 ctgaagaggc agtgaaatgg ggtgaatcat ttgacaaact gctttcccat agagatggac
      181 tagaggcttt taccagattt cttaaaactg aattcagtga agaaaatatt gaattttgga
      241 tagcctgtga agatttcaag aaaagcaagg gacctcaaca aattcacctt aaagcaaaag
      301 caatatatga gaaatttata cagactgatg ccccaaaaga ggttaacctt gattttcaca
      361 caaaagaagt cattacaaac agcatcactc aacctaccct ccacagtttt gatgctgcac
      421 aaagcagagt gtatcagctc atggaacaag acagttatac acgttttctg aaatctgaca
      481 tctatttaga cttgatggaa ggaagacctc agagaccaac aaatcttagg agacgatcac
      541 gctcatttac ctgcaatgaa tgacagtaaa ggtggatacg gatccgaatt cgagctccgt
      601 cgacaagctt gcggccgcac tcgagcacca ccaccaccac cactgagatc cggctgctaa
      661 caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc
      721 ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg
      781 attggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
      841 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccc gcctcctttc gctttctttc
      901 cctt
//
