LOCUS       sequence-52497-e         654 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 654)
  TITLE     Chin Lab P85 plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 654)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLenti4/V5-DEST-P85(D560Y)
FEATURES             Location/Qualifiers
     source          1..654
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    90..114
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 aatcgagacc gaggagaggg ttagggatag gcttaccttc gaaccgcggg ccctctagac
       61 tcgagcggcc gccactgtgc tggatatcaa ccactttgta caagaaagct gggtcggcgc
      121 gcccaccctt aattaatcat cgcctctgct gtgcatatac tgggtaggct agtgtgacat
      181 tgagggagtc gttgtgctgc acaagggagg tgtgttggta atgtagcacc agttctttca
      241 gagagctgta caagttatag ggctcggcaa agccatagcc agttgctgtt ttgtttatga
      301 cacaatgctt tacttcgccg tccaccacta cagagcaggc atagcagccc tgtttactgc
      361 tctcccggac aagaaaagtg ccatctcgct tccctcgcaa caggttttca gctttgtttc
      421 ggttgctgct tccaacattc catgtcttct catcatgatg gggcaaatct tcatcatctt
      481 ccaccagtga atattggtct tcagtgtttt cattgcccaa ccactcgttc aacttctttt
      541 gccgaacacc tttttgagtc aaccacatca agtattggtc tctcgtcttt ctcagctgga
      601 taaggtctgg tttaatgctg ttcatacgtt tgtaaatttc tcgatactca gctg
//
